the chick embryo chorioallantoic membrane as an in vivo assay to study antiangiogenesis小鸡胚胎绒毛膜尿囊的膜作为体内试验研究抗血管.pdf

Pharmaceuticals 2010, 3, 482-513; doi:10.3390/ph3030482 OPEN ACCESS pharmaceuticals ISSN 1424-8247 /journal/pharmaceuticals Review The Chick Embryo Chorioallantoic Membrane as an In Vivo Assay to Study Antiangiogenesis Domenico Ribatti Department of Human Anatomy and Histology, University of Bari Medical School, Piazza G. Cesare, 11, Policlinico 70124, Bari, Italy; E-Mail: ribatti@anatomia.uniba.it; Tel.: +39-080-547-8240; Fax: +39-080-547-8310. Received: 12 January 2010; in revised form: 29 January 2010 / Accepted: 2 March 2010 / Published: 8 March 2010 Abstract: Antiangiogenesis, e.g., inhibition of blood vessel growth, is being investigated as a way to prevent the growth of tumors and other angiogenesis-dependent diseases. Pharmacological inhibition interferes with the angiogenic cascade or the immature neovasculature with synthetic or semi-synthetic substances, endogenous inhibitors or biological antagonists. The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane, which serves as a gas exchange surface and its function is supported by a dense capillary network. Because its extensive vascularization and easy accessibility, CAM has been used to study morphofunctional aspects of the angiogenesis process in vivo and to study the efficacy and mechanism of action of pro- and anti-angiogenic molecules. The fields of application of CAM in the stud