substituted pyrazinecarboxamides as abiotic elicitors of flavolignan production in silybum marianum (l.) gaertn cultures in vitro代替pyrazinecarboxamides flavolignan生产的非生物细胞内silybum marianum(l).pdf

Molecules 2010, 15, 331-340; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Substituted Pyrazinecarboxamides as Abiotic Elicitors of Flavolignan Production in Silybum marianum (L.) Gaertn Cultures in Vitro Lenka Tůmová 1,*, Jiří Tůma 2, Klara Megušar 1 and Martin Doležal 1 1 Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic; E-Mails: martin.dolezal@faf.cuni.cz (M.D.); klara.megusar@ (K.M.) 2 Department of Biology, Faculty of Education, University of Hradec Králové, Víta Nejedlého 573, 500 03 Hradec Králové, Czech Republic; E-Mail: jiri.tuma@uhk.cz (J.T.) * Author to whom correspondence should be addressed; E-Mail: lenka.tumova@faf.cuni.cz. Received: 27 November 2009; in revised form: 11 January 2010 / Accepted: 12 January 2010 / Published: 14 January 2010 Abstract: Substituted pyrazinecarboxamides markedly influenced production of flavonolignans in Silybum marianum callus and suspension cultures. In this study the effect of two compounds, N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide (1) and N-(3-iodo- 4-methylphenyl)-5-tert-butyl-pyrazine-2-carboxamide (2), as abiotic elicitors on flavono- lignan production in callus culture of S. marianum was investigated. Silymarin complex compounds have hepatoprotective, anticancer and also hypocholesterolemic activity. In vitro flavonolignan concentration in cells is very low