the expression profiling of the lipoxygenase (lox) family genes during fruit development, abiotic stress and hormonal treatments in cucumber (cucumis sativus l.)表达式分析脂氧合酶(lox)家族基因在水果的发展过程中,非生物压力和荷尔蒙治疗黄瓜(cucumis巨大).pdf

Int. J. Mol. Sci. 2012, 13, 2481-2500; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article The Expression Profiling of the Lipoxygenase (LOX) Family Genes During Fruit Development, Abiotic Stress and Hormonal Treatments in Cucumber (Cucumis sativus L.) Xue-Yong Yang *, Wei-Jie Jiang * and Hong-Jun Yu Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China; E-Mail: yuhj@ * Author to whom correspondence should be addressed; E-Mail: xueyongyang1982@ (X.-Y.Y.); jiangwj@ (W.-J.J.); Tel.: +86-10 Received: 23 December 2011; in revised form: 8 February 2012 / Accepted: 15 February 2012 / Published: 22 February 2012 Abstract: Lipoxygenases (LOXs) are non-haem iron-containing dioxygenases that catalyse oxygenation of polyunsaturated fatty acids and lipids to initiate the formation of a group of biologically active compounds called oxylipins. Plant oxylipins play important and diverse functions in the cells. In the current study, expression analysis during cucumber development using semi-quantitative RT-PCR revealed that 13 of 23 CsLOX genes were detectable, and were tissue specific or preferential accumulation. In total, 12 genes were found to be differentially expressed during fruit development and have different patterns of expression in exocarp, endocarp and pulp at day 5 after anthesis. The expression analysis of