corticolimbic expression of trpc4 and trpc5 channels in the rodent braincorticolimbic表达式trpc4和trpc5频道的啮齿动物的大脑.pdf

Corticolimbic Expression of TRPC4 and TRPC5 Channels in the Rodent Brain 1 2 1 1 1 Melissa A. Fowler , Kyriaki Sidiropoulou , Emin D. Ozkan , Christopher W. Phillips , Donald C. Cooper * 1 Psychiatry Department, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 2 Computational Biology Lab, Institute of Molecular Biology and Biotechnology (IMBB)-Foundation for Research and Technology (FORTH), Vassilika Vouton, Heraklio, Greece The canonical transient receptor potential (TRPC) channels are a family of non-selective cation channels that are activated by increases in intracellular Ca2+ and G /phospholipase C-coupled receptors. We used quantitative real-time PCR, in situ q hybridization, immunoblots and patch-clamp recording from several brain regions to examine the expression of the predominant TRPC channels in the rodent brain. Quantitative real-time PCR of the seven TRPC channels in the rodent brain revealed that TRPC4 and TRPC5 channels were the predominant TRPC subtypes in the adult rat brain. In situ hybridization histochemistry and immunoblotting further resolved a dense corticolimbic expression of the TRPC4 and TRPC5 channels. Total protein expression of HIP TRPC4 and 5 proteins increased throughout development and peaked late in adulthood (6–9 weeks). In adults, TRPC4 expression was high throughout the frontal cortex, lateral septum (LS), pyramidal cell layer of the hippocampus (HIP), dentate gyrus (DG), and ventral subiculum (vSUB). TRPC5 was highly expressed in the frontal cortex, pyramidal cell layer of the HIP, DG, and hypothalamus. Detailed examination of frontal cortical layer mRNA expressi