some ethylene biosynthesis and ap2erf genes reveal a specific pattern of expression during somatic embryogenesis in hevea brasiliensis一些乙烯生物合成和ap2erf揭示特定基因表达模式在橡胶树体细胞胚胎发生取代巴西橡胶树.pdf

Piyatrakul et al. BMC Plant Biology 2012, 12:244 /1471-2229/12/244 RESEARCH ARTICLE Open Access Some ethylene biosynthesis and AP2/ERF genes reveal a specific pattern of expression during somatic embryogenesis in Hevea brasiliensis 1,2 1,3 1 1 1 1 Piyanuch Piyatrakul , Riza-Arief Putranto , Florence Martin , Maryannick Rio , Florence Dessailly , Julie Leclercq , 1 1 1* Jean-François Dufayard , Ludovic Lardet and Pascal Montoro Abstract Background: Ethylene production and signalling play an important role in somatic embryogenesis, especially for species that are recalcitrant in in vitro culture. The AP2/ERF superfamily has been identified and classified in Hevea brasiliensis. This superfamily includes the ERFs involved in response to ethylene. The relative transcript abundance of ethylene biosynthesis genes and of AP2/ERF genes was analysed during somatic embryogenesis for callus lines with different regeneration potential, in order to identify genes regulated during that process. Results: The analysis of relative transcript abundance was carried out by real-time RT-PCR for 142 genes. The transcripts of ERFs from group I, VII and VIII were abundant at all stages of the somatic embryogenesis process. Forty genetic expression markers for callus regeneration capacity were identified. Fourteen markers were found for proliferating calli and 35 markers for calli at the end of the embryogenesis induction phase. Sixteen markers discriminated between normal and abnormal embryos and, lastly, there were 36 markers of conversion into plantlets. A phylogenetic analysis comparing the sequences of the AP2 domains of