乳酸菌发酵剂高密度培养的研究.pdf

5 No. 5 CHINA CONDIMENT 2004 5 May. 2004 : 1000- 9973(2004) 05- 0017- 05 熊晓辉, 于修 , 熊强, 陆利霞 ( , 210009) : 研究了乳酸菌生长繁殖的环境条件( 温度接种量起始pH 等) 和培养基组成( 氮源 源缓 冲盐等) , 优化确定了乳酸菌发酵剂的适宜培养条件为: 起始 pH 值为6. 5, 培养温度为37, 培养 基配比为麦芽糖乳糖( 11) 2% 牛肉膏 1. 0%缓冲盐A0. 5%NaCl0. 25% MgSO 0. 1%, 接种量 4 4% , 进一步探索了半连续法进行高密度培养, 结合优化的培养条件, 可使乳酸菌的液体发酵活菌密 12 度至1. 1 10 CFU/ m : 乳酸菌; 发酵剂; 高密度培养 :TS201. 5 :A High cell density cult ure of lact ic acid bacteria starter XIONG Xiaohui, YU Xiujian, XIONG Qiang, U iXia ( College of ife Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China) Abstract: This paper studied on the high cell density culture of L actobaci ll us sp p . , and discussed the effects of cultural condition (temperature, inoculation capacity and initial pH) and media composition. The optimal culturing conditions were developed , following: 1% maltose, 1% lactose, 1.0% beef ex tract, 0. 5% buffer salt A, 0. 25% sodium chloride, 0. 1%magnesium sulfate, pH6. 5, 4% inoculation capacity. T hen high cell density culture wasexplored with one semi- continuousway and obtained the 12 cells population over 1. 1 10 CFU/ m for 16 hours based on the optimal condition. Key words: actic acid bacteria; starter; high cell density culture ) , , , , , , , , [ 1, 2] [ 1, 3, 4] [ 1, 4] (