cytotoxicity of phenol red in toxicity assays for carbon nanoparticles细胞毒性的酚红为碳纳米粒子毒性分析.pdf

Int. J. Mol. Sci. 2012, 13, 12336-12348; doi:10.3390/ijms131012336 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Cytotoxicity of Phenol Red in Toxicity Assays for Carbon Nanoparticles Ying Zhu 1,*, Xiaoyong Zhang 1, Jianhua Zhu 2, Qunfen Zhao 3, Yuguo Li 1, Wenxin Li 1,*, 1 1 Chunhai Fan and Qing Huang 1 Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; E-Mails: xiaoyongzhang1980@ (X.Z.); yuguo98@ (Y.L.); fanchunhai@ (C.F.); huangqing@ (Q.H.) 2 School of Pharmacy, Fudan University, Shanghai 200032, China; E-Mail: jhzhu@ 3 Faculty of Life Science and Bioengineering, Ningbo University, Ningbo 315211, China; E-Mail: zhaoqunfen@ * Authors to whom correspondence should be addressed; E-Mails: zhuying@ (Y.Z.); liwenxin@ (W.L.); Tel.: +86-21-3919-4007 (Y.Z.); +86-21-3919-4689 (W.L.); Fax: +86-21-3919-4022 (Y.Z. W.L.). Received: 16 August 2012; in revised form: 18 September 2012 / Accepted: 19 September 2012 / Published: 26 September 2012 Abstract: To explore the novel properties of carbon nanoparticles (CNPs) in nanotoxicity assays, the adsorption of phenol red (a pH indicator for culture medium) by multi-walled carbon nanotubes (MWNTs) and three kinds of carbon blacks (CBs) with nanosize, and its effects on cytotoxicity were studied. Results indicated that the phenol red