biological toxicity and inflammatory response of semi-single-walled carbon nanotubessemi-single-walled碳纳米管的生物毒性和炎症反应.pdf

Biological Toxicity and Inflammatory Response of Semi- Single-Walled Carbon Nanotubes 1 2 3 3 3 2 Eun-Jung Park , Jinkyu Roh , Soo Nam Kim , Min-Sung Kang , Byoung-Seok Lee , Younghun Kim , Sangdun Choi1* 1 Department of Molecular Science and Technology, Ajou University, Suwon, Korea, 2 Department of Chemical Engineering, Kwangwoon University, Seoul, Korea, 3 Inhalation Toxicology Center, Korea Institute of Toxicology, Jeongeup, Korea Abstract The toxicological studies on carbon nanotubes (CNTs) have been urgently needed from the emerging diverse applications of CNTs. Physicochemical properties such as shape, diameter, conductance, surface charge and surface chemistry of CNTs gained during manufacturing processes play a key role in the toxicity. In this study, we separated the semi-conductive components of SWCNTs (semi-SWCNTs) and evaluated the toxicity on days 1, 7, 14 and 28 after intratracheal instillation in order to determine the role of conductance. Exposure to semi-SWCNTs significantly increased the growth of mice and significantly decreased the relative ratio of brain weight to body weight. Recruitment of monocytes into the bloodstream increased in a time-dependent manner, and significant hematological changes were observed 28 days after exposure. In the bronchoalveolar lavage (BAL) fluid, secretion of Th2-type cytokines, particularly IL-10, was more predominant than Th1-type cytokines, and expression of regulated on activation normal T cell expressed and secreted (RANTES), p53, transforming growth factor (TGF)-b, and inducible nitric oxide synthase (iNOS) increased in a time-dependent manner. Fibrotic histopathological changes peaked o