solubilization of single-walled carbon nanotubes with single- stranded dna generated from asymmetric pcr溶解的单壁碳纳米管和单-链dna产生不对称pcr.pdf

Int. J. Mol. Sci. 2007, 8, 705-713 International Journal of Molecular Sciences ISSN 1422-0067 © 2007 by MDPI /ijms/ Communication Solubilization of Single-walled Carbon Nanotubes with Single- stranded DNA Generated from Asymmetric PCR Zhiqiang Liang 1, 2, Ruojun Lao 2, Jing Wang 1, 2, Yongbiao Liu 3, Lihua Wang 2, Qing Huang 2 , Shiping Song 2,*, Genxi Li 1, * and Chunhai Fan 2 1 Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, P. R. China; E-mail: genxili@ (for Genxi Li) 2 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China; E-mail: spsong@ (for Shiping Song) 3 The People’s Hospital of Jiangsu Province, Nanjing 210029, P. R. China * Author to whom correspondence should be addressed. Tel: +86 21 Fax: +86 21 E-mail: spsong@ Received: 31 May 2007; in Revised Form: 29 June 200 7 / Accepted: 13 July2007 / Published: 23 July 2007 Abstract: Carbon nanotubes (CNTs) can be effectively dispersed and functionalized by wrapping with long single-stranded DNA (ssDNA) synthesized by asymmetric PCR. The ssDNA-CNTs attached on surface of glass carbon electrode made it possible for electrochemical analysis and sensing, which was demonstrated by reduction of H O on 2 2 hemoglobin/ss