surface-modified gold nanoparticles with folic acid as optical probes for cellular imaging基团,金纳米粒子与叶酸作为细胞成像的光学探针.pdf

Sensors 2008, 8, 6660-6673; DOI: 10.3390/s8106660 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Surface-Modified Gold Nanoparticles with Folic Acid as Optical Probes for Cellular Imaging Shiao-Wen Tsai 1,*, Jiunn-Woei Liaw 2, Fu-Yin Hsu 3,*, Yi-Yun Chen 1, Mei-Jhih Lyu 1 and 4 Ming-His Yeh 1 Institute of Biochemical and Biomedical Engineering, Chang Gung University 259 Wen-Hwa 1st Rd., Kwei-Shan, Tao-Yuan, 333, Taiwan 2 Department of Mechanical Engineering, Chang Gung University, 259 Wen-Hwa 1st Rd., Kwei- Shan, Tao-Yuan, 333, Taiwan. E-Mail: markliaw@.tw 3 Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Taiwan Keelung, Taiwan 4 FEOL Module Department / Platform 2 Division RD; Taiwan Semiconductor Manufacturing Company, Ltd. E-Mail: msyehb@ * Authors to whom correspondence should be addressed; E-Mails: swtsai@.tw; fyhsu@.tw Received: 24 July 2008; in revised form: 21 September 2008 / Accepted: 21 October 2008 / Published: 24 October 2008 Abstract: In this study, we demonstrate that the uptake rate of the surface-modified gold nanoparticles (GNPs) with folic acid by specific cells can be increased significantly, if the membranes of these cells have sufficient folic-acid receptors. Two human breast cancer cell lines were studied; one is MDA-MB-435S cell, and the other T-47D cell. The expression of the folic acid receptors of the former is much higher than that of the latter. These cells were incubated