deep and clear optical imaging of thick inhomogeneous samples深刻而清晰的光学成像厚不均匀样本.pdf

Deep and Clear Optical Imaging of Thick Inhomogeneous Samples 1,2. ´ ¨ 1,2. 3 1,2 ´ 1,2 Raphael Jorand , Gwenaele Le Corre , Jordi Andilla , Amina Maandhui , Celine Frongia , ´ 1,2, Bernard Ducommun1,2,4, Corinne Lorenzo1,2* Valerie Lobjois 1 University of Toulouse, ITAV-UMS3039, Toulouse, France, 2 CNRS, ITAV-UMS3039, Toulouse, France, 3 ICFO, Institut de Ciences Fotonique, Mediterraneen Technology Park, Castelldefels, Barcelona, Spain, 4 CHU de Toulouse, Toulouse, France Abstract Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (WAO). Our WAOSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point source emitters. We demonstrate the use of this WAOSPIM method to correct distortions in three-dimensional biological imaging and to improve the quality of images from deep within thick inhomogeneous samples. Citation: Jorand R, Le Corre G, Andilla J, Maandhui A, Frongia C, et al. (2012) Deep and Clear Optical Imaging of Thick Inhomogeneous Samples. PLoS ONE 7(4): e35795. doi:10.1371/journal.pone.0035795 Editor: Sharon Gerecht, Johns Hopkins University, United States of America Received November 30, 2011; Accepted March 21, 2012; Published April 25, 2012 Copyright: 2012 Jorand et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribut