temporal trends in the secondary metabolite production of the sponge aplysina aerophoba时间趋势的次生代谢物生产海绵aplysina aerophoba.pdf

Mar. Drugs 2012, 10, 677-693; doi:10.3390/m OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Temporal Trends in the Secondary Metabolite Production of the Sponge Aplysina aerophoba Oriol Sacristán-Soriano 1, Bernard Banaigs 2 and Mikel A. Becerro 3,* 1 Center for Advanced Studies of Blanes (CEAB-CSIC), Accés a la Cala St. Francesc 14, Blanes 17300, Girona, Spain; E-Mail: osacristan@ceab.csic.es or osacriso7@ 2 Environmental and Biomolecular Chemistry Laboratory, University of Perpignan, Via Domitia, 52 Paul Alduy Ave., Perpignan Cedex 66860, France; E-Mail: banaigs@univ-perp.fr 3 Natural Products and Agrobiology Institute (IPNA-CSIC), Avda. Astrofísico Francisco Sánchez 3, La Laguna, Tenerife 38206, Spain * Author to whom correspondence should be addressed; E-Mail: m.becerro@csic.es; Tel.: +34-922-256-847; Fax: +34-922-260-135. Received: 24 February 2012; in revised form: 12 March 2012 / Accepted: 14 March 2012 / Published: 23 March 2012 Abstract: Temporal changes in the production of secondary metabolites are far from being fully understood. Our study quantified, over a two-year period, the concentrations of brominated alkaloids in the ectosome and the choanosome of Aplysina aerophoba , and examined the temporal patterns of these natural products. Based on standard curves, we quantified the concentrations of aerophobin-2, aplysinamisin-1, and isofistularin-3: three of the four major peaks obtained through chemical