spontaneous periodontitis development in diabetic rats involves an unrestricted expression of inflammatory cytokines and tissue destructive factors in the absence of major changes in commensal oral microbiota英文论文.pdf

Hindawi Publishing Corporation Experimental Diabetes Research Volume 2012, Article ID 356841, 10 pages doi:10.1155/2012/356841 Research Article Spontaneous Periodontitis Development in Diabetic Rats Involves an Unrestricted Expression of Inflammatory Cytokines and Tissue Destructive Factors in the Absence of Major Changes in Commensal Oral Microbiota 1 1 1 ´ 2 Marcela Claudino, Gabriela Gennaro, Tania Mary Cestari, Cesar Tadeu Spadella, Gustavo Pompermaier Garlet,1 and Gerson Francisco Assis1 1 Department of Biological Sciences, School of Dentistry of Bauru, Sao Paulo University (FOB/USP), Al. Dr Octavio Pinheiro Brisolla, 9-75, 17012-901 Bauru, SP, Brazil ´ 2 Department of Surgery and Orthopedics, School of Medicine of Botucatu, Sao Paulo State University (UNESP), 18618-970 Botucatu, SP, Brazil Correspondence should be addressed to Marcela Claudino, marcelaclaudino@ Received 5 January 2012; Accepted 14 February 2012 Academic Editor: Shi Fang Yan Copyright © 2012 Marcela Claudino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Diabetes mellitus is a heterogeneous group of disorders, in which hyperglycemia is a main feature. The objective was to evaluate the involvement of RAGE, inflammatory cytokines, and metalloproteinases in spontaneous periodontitis triggered by diabetes induction. Immunohistochemical procedures for MMP-2, MMP-9, TNF-α, IL-1β, IL-6, RANKL, and RAGE were performed in rats after 1, 3, 6, 9, and 12 mont