single intramammary infusion of recombinant bovine interleukin-8 at dry-off induces the prolonged secretion of leukocyte elastase, inflammatory lactoferrin-derived peptides, and interleukin-8 in dairy cows英文论文.pdf

Hindawi Publishing Corporation Veterinary Medicine International Volume 2012, Article ID 172072, 8 pages doi:10.1155/2012/172072 Research Article Single Intramammary Infusion of Recombinant Bovine Interleukin-8 at Dry-Off Induces the Prolonged Secretion of Leukocyte Elastase, Inflammatory Lactoferrin-Derived Peptides, and Interleukin-8 in Dairy Cows Atsushi Watanabe,1 Jiro Hirota,2 Shinya Shimizu,2 Shigeki Inumaru,3 and Kazuhiro Kimura4 1 Dairy Hygiene Research Division, Hokkaido Research Station, National Institute of Animal Health (NIAH), National Agriculture and Food Research Organization (NARO), 4 Hitsujigaoka, Sapporo 062-0045, Japan 2 Intellectual Property and Technology Management Section, NIAH, NARO, 3-1-1 Kannnondai, Tsukuba 305-0856, Japan 3 Pathology and Pathophysiology Research Division, NIAH, NARO, 3-1-1 Kannnondai, Tsukuba 305-0856, Japan 4 Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Correspondence should be addressed to Atsushi Watanabe, awata@affrc.go.jp Received 11 April 2012; Accepted 25 June 2012 Academic Editor: Jyoji Yamate Copyright © 2012 Atsushi Watanabe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A single intramammary infusion of recombinant bovine interleukin-8 (IL-8) at 50 μg/quarter/head, but not 10 μg/quarter/head, induced clinical mastitis in three of four cows during the dry-off period, resulting in an elevated rectal temperature, redness and swelling of the mammary gland, extensive polymorphonuclear leukocyte (PMNL) infiltration, and milk clot forma