bio-imaging of colorectal cancer models using near infrared labeled epidermal growth factor能结直肠癌模型使用近红外标记表皮生长因子.pdf

Bio-Imaging of Colorectal Cancer Models Using Near Infrared Labeled Epidermal Growth Factor 1 1 1 1 1 2 Gadi Cohen , Shimon Lecht , Hadar Arien-Zakay , Keren Ettinger , Orit Amsalem , Mor Oron-Herman , 1 3 1 4 1 Eylon Yavin , Diana Prus , Simon Benita , Aviram Nissan , Philip Lazarovici * 1 School of Pharmacy Institute for Drug Research, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel, 2 Advanced Technology Center, The Chaim Sheba Medical Center, Tel-Hashomer, Israel, 3 Department of Pathology and Surgical Oncology Laboratory, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem, Israel, 4 Department of Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem, Israel Abstract Novel strategies that target the epidermal growth factor receptor (EGFR) have led to the clinical development of monoclonal antibodies, which treat metastatic colorectal cancer (mCRC) but only subgroups of patients with increased wild type KRAS and EGFR gene copy, respond to these agents. Furthermore, resistance to EGFR blockade inevitably occurred, making future therapy difficult. Novel bio-imaging (BOI) methods may assist in quantization of EGFR in mCRC tissue thus complementing the immunohistochemistry methodology, in guiding the future treatment of these patients. The aim of the present study was to explore the usefulness of near infrared-labeled EGF (EGF-NIR) for bio-imaging of CRC using in vitro and in vivo orthotopic tumor CRC models and ex vivo human CRC tissues. We describe the preparation and characterization of EGF-NIR and investigate binding, using BOI of a pan