cord blood stem cells inhibit epidermal growth factor receptor translocation to mitochondria in glioblastoma脐带血干细胞抑制表皮生长因子受体易位在胶质母细胞瘤线粒体.pdf

Cord Blood Stem Cells Inhibit Epidermal Growth Factor Receptor Translocation to Mitochondria in Glioblastoma 1 1 1 3 2,4 Venkata Ramesh Dasari , Kiran Kumar Velpula , Kiranmai Alapati , Meena Gujrati , Andrew J. Tsung * 1 Departments of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois, United States of America, 2 Department of Neurosurgery, University of Illinois College of Medicine at Peoria, Peoria, Illinois, United States of America, 3 Department of Pathology, University of Illinois College of Medicine at Peoria, Peoria, Illinois, United States of America, 4 Illinois Neurological Institute, University of Illinois College of Medicine at Peoria, Peoria, Illinois, United States of America Abstract Background: Overexpression of EGFR is one of the most frequently diagnosed genetic aberrations of glioblastoma multiforme (GBM). EGFR signaling is involved in diverse cellular functions and is dependent on the type of preferred receptor complexes. EGFR translocation to mitochondria has been reported recently in different cancer types. However, mechanistic aspects of EGFR translocation to mitochondria in GBM have not been evaluated to date. Methodology/Principle Findings: In the present study, we analyzed the expression of EGFR in GBM-patient derived specimens using immunohistochemistry, reverse-transcription based PCR and Western blotting techniques. In clinical samples, EGFR co-localizes with FAK in mitochondria. We evaluated this previous observation in standard glioma cell lines and