1111-110308鸭LXRa基因cDNA的克隆及序列分析(修改).doc

[文章编号]1001-3601（2011）00- -00 -0 *鸭LXRα基因cDNA的克隆及序列分析 张依裕，陈 伟（1.贵州大学 动物科学学院，贵州 贵阳 550025，贵州 贵阳 550025)[摘 要] LXRα在鸭的脂代谢过程中起着重要作用。本研究以兴义鸭肝脏组织总RNA为模板，采用RT-PCR法对LXRα基因进行扩增测序，分析其序列特征，结果表明鸭LXRα基因扩增产物克隆拼接的序列全长为1412bp（GenBank登录号：FJ966078），开放阅读框1230bp，包含起始密码子ATG和终止密码子TGA，编码409个氨基酸残基的LXRα锌指蛋白，含有17个磷酸化位点3个糖基化位点2个锌指结构（P-盒和D-盒）1个配体结合区（LBD）无跨膜螺旋，表现出典型的核激素受体超家族的结构特征。鸭LXRα蛋白与哺乳动物和鱼类的LXRα蛋白的同源性为73%～76%，与禽类的同源性高达97%左右，说明LXRα基因在系统进化过程中具有很高的保守性。 [关键词] 鸭；肝X受体α；RT-PCR；克隆；序列分析 [中图分类号] S835 [文献标识码]A Cloning and Sequence Analysis of cDNA Encoding duck Liver X Receptor alpha ZHANG Yi-yu1,2，CHEN Wei,2，ZHANG Qi-lin1，Li Qian1 (1. College of Animal Science，Guizhou University，Guiyang 550025，China; .Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China) Abstract: Liver X receptor alpha(LXRα) plays an important role in lipid metabolism. The cDNA of liver X receptor alpha(LXRα) gene was cloned from total RNA of liver in Xingyi duck by RT-PCR，and analysed its sequence feature. The results indicated that the duck LXRα cDNA was 1412bp in length (GenBank accession number: FJ966078)，the open reading frame 1230bp，including the start codon ATG and stop codon TGA，and encoded a deduced LXRα protein with 409 amino acids residues. The analysis of deduced amino acids sequence showed that LXRα was a zinc finger proteins with 17-phosphorlated sites, 3-glycosylated sites, 2-zinc finger domains(P-box and D-box) and a hydrophobic ligand-binding domain (LBD) without transmenbrane helices, and displayed the common structural features of the nuclear hormone receptor superfamily. The identity of amino acids sequence of duck LXRα with mammals and fish were about 73% to 76%. However，it was about 97% with that of avian. The results suggested that the LXRα gene was highly conserved in evolution. Key words: duck; liver X receptor alpha; RT-PCR; clone; sequence analysis 肝X受体（Liver X receptors，LXRs