小菜蛾碱性磷酸酶基因cDNA片段的克隆及其序列分析-应用昆虫学报.PDF

昆虫知识 Chinese Bulletin of Entomology 20 10 ，47 (2 ):270 ～ 274 殤檻檻檻檻檻殤 檻 檻 檻 研究论文檻 殤檻檻檻檻檻殤 小菜蛾碱性磷酸酶基因cDNA 片段的 克隆及其序列分析* 1 ，2  1 2 2 2  刘 洁 文礼章 王少丽 吴青君 张友军 (1. 湖南农业大学生物安全科学技术学院 长沙 4 10 128 ;2 . 中国农业科学院蔬菜花卉研究所 北京 10008 1) Cloning and sequence analysis of alkaline phosphatase gene in the diamondback moth ，Plutella 1 ，2  1 2 3 2  xy lostella. LIU Jie ，WEN Li-Zhang ，WANG Shao-Li ，WU Qing-Jun ，ZHANG You-Jun (1. Hunan Agricultural University College of Bio-saf ety Sciences and Technology ，Hunan Agricultural University ，Changsha 4 10 128 ，China ;2. Institute of Vegetables and Flowers ，Chinese A cademy of Agricultural Sciences ，Beijing 10008 1 ，China) Abstract Alkaline phosphatase genes were cloned from the resistant and susceptible strains of Plutella xylostella (L . )through RT -PCR technique using degenerate primers and sequenced . The cDNA length was 403 bp and the sequence was confirmed to be the alkaline phosphatase gene by blast alignment (BLAST ). The nucleotide similarity of the resistant and susceptible strains was 99. 5 % and the amino acids identity was 99 % . Compared with the susceptible strain ，18 nucleotides were changed in the resistant strain ，resulting in one amino acid changed (V to T ). These results are helpful for further study of the full sequence and function of alkaline phosphatase gene in P. xylostella. Key words Plutella xylostella ，alkaline phosphatase ，cloning ，sequence analysis DNAman ， (RT -PCR ) Plutella 摘 要 利用 设计简并引物 通过反转录一多聚酶链式反应 克隆小菜蛾 xylostell