蛋白质内含肽的研究进展.PDF

6 4 ( ) 生命科学研究 Vol. 6 No. 4 ( Suppl. ) 2002 12 Life Science Research Dec. 2002 X 郝岗平, 杨 清 ( , 210095) : 蛋白质内含肽是能够自我剪接的 一段多肽链. 它的发 不仅在理论上丰富了遗传信息翻译后加工的内 容, 而且在蛋白质纯化的实践方面有着广泛的应用前景. 主要对蛋白质内含肽的剪接机制结构特征核酸内切 酶活性以及应用方面的研究进展作 一概述. : 蛋白质内含肽 自我剪接 归巢核酸内切酶 : Q591. 2 : A : 1007- 7847 ( 2002) S1- 0136- 04 Advances in Intein HAO Gang-ping, YANG Qing ( College of Lfie Science , Nanj ing Agricultural University , Nanjing 210095, Jiangsu, China) Abstract: Inteins are internal segment peptides which can self- splicing at the protein level. Its discovery not only enriches the content of the process that the gene transfers its information but also can be used in protein purif icat ion. The progress in the mechanism of self- splicing, structural characterist ic, homing endonuclease and the applicat ion of intein were summarized . Key words: intein sel-f splicing of intein homing endonuclease ( Lfie Science Research, 2002, 6( Suppl) : 136~ 139) ( intein) . . DNA , . , 1 , , , 1990 Kane ( Saccharomyces . 1990 , + cerevisiae) H -ATPase 69 kD( 1 D= 1 u= 1. 6 . 605 402 @ 10- 27 kg) vma1 RNA , , . RNA (