响应面法优化超声辅助提取佛手瓜多糖工艺.pdf

山东农业大学学报( 自然科学版),2017,48(2):171-177 VOL.48 NO.2 2017 Journal of Shandong Agricultural University ( Natural Science Edition ) doi:10.3969/j .issn.1000-2324.20 17.02.003 响应面法优化超声辅助提取佛手瓜多糖工艺 陈健旋 漳州职业技术学院 食品与生物工程系; 农产品深加工及安全福建省高校应用技术工程中心, 福建 漳州 363000 摘 要: 本文以佛手瓜为原料，采用响应面法优化超声辅助提取佛手瓜多糖。在单因素试验基础上，运用 Box-Behnken 中心组合实验和响应面法考察了液料比、超声功率、超声温度、超声时间四个因素对佛手瓜多糖得率的影响，并优 化了提取工艺。结果表明最佳的工艺条件为：液料比30 mL ·g-1、超声功率 245 W 、超声温度 65 ℃，超声 40 min 。在 此条件下佛手瓜多糖得率的预测值为 3.319%，验证实验值为 3.288%，其相对误差为 0.934% 。 关键词: 佛手瓜; 超声; 多糖 中图法分类号:TS255;R93 文献标识码:A 文章编号:1000-2324(2017)02-0 171-07 Polysaccharides Extracted from Sechium edule (Jacq.) Swartz by Response SurfaceMethodologywithUltrasonicOptimization CHEN Jian-xuan Applied Technology Engineering Center of Fujian University for Further Processing and Safety of Agricultural Products, Departmentof FoodandBiology Engineering/ZhangzhouInstituteof Technology,Zhangzhou 363000, China Abstract: The ultrasonic-assisted extraction conditions of Polysaccharides from Sechium edule (Jacq.) Swartz were optimized by response surface methodology. Based on the single-factor experiments, the effects of four variables on Polysaccharides yield and the optimum process were evaluated by Box-Behnken central composite experimental design and response surface methodology analysis. The results showed the optimum conditions were the ratio of liquid-to-material 30 mL ·g-1, ultrasonic power 245 W, ultrasonic temperature 65 ℃, ultrasonic time 40 min, under these optimized conditions the yield of polysaccharides could be up to 3.288% and the relative error was 0.934%. Keywords:Sechium edule (Jacq.) Swartz; Ultrasonic; Polysaccharide 佛手瓜 （Sechium edule (Jacq.) Swartz ）原产于西印度群岛、墨西哥及中美洲热带，是葫芦科梨 瓜属的一种珍稀瓜类蔬菜，于 19 世纪初传入我国，目前在我国的广东、福建、浙江、云南、山东等 地有大量的种植[1,2] 。佛手瓜生命力旺盛，适应性强，是一种优质高产的“无公害”蔬菜。佛手瓜营养 丰富，富含人体所需胡萝 卜素及镁、铁、磷、钙、锌等多种矿物质，还含有各种氨基酸等天然活性