GC_MS法分析白花丹参不同部位挥发性成分.pdf

GC-MS 法分析白花丹参不同部位挥发性成分Δ 1＊ 2 3 3# 4 冯 蕾 ，冀海伟 ，王德才 ，王健美 ，柳仁民 （1.泰山医学院动脉粥样硬化研究所，泰安市 271016；2.泰山医 学院化学与化学工程学院，泰安市 271016；3.泰山医学院生物科学系，泰安市 271016；4.聊城大学化学化工 学院，聊城市 252059） 中图分类号 R284.1；R931.6 文献标识码 A 文章编号 1001-0408（2010）39-3706-04 摘 要 目的：研究白花丹参不同部位挥发油化学成分并进行比较。方法：采用水蒸气蒸馏法提取白花丹参根、茎、叶及花的挥发 油，应用气相色谱-质谱（GC-MS ）联用技术对各部位挥发油进行分析，利用面积归一化法分别计算各成分的相对质量分数。结 果：从白花丹参根、茎、叶、花的挥发油中分别鉴定了39、33、21、28种成分。白花丹参根、茎、叶、花挥发油中主要化学成分差别很 大，除大根香叶烯D 为共有，且在茎、叶、花中相对质量分数都较高外，其它主要成分均不同。结论：本试验结果可为白花丹参的进 一步开发利用提供依据。 关键词 白花丹参；挥发油；化学成分；气相色谱-质谱联用法 Analysis of Volatile Constituents from Different Parts of Salvia miltiorrhiza by GC-MS FENG Lei （Institute of Atherosclerosis ，Taishan Medical University ，Taian 271016，China ） JI Hai-wei （College of Chemistry and Chemical Engineering ，Taishan Medical University ，Taian 271016，China ） WANG De-cai ，WANG Jian-mei （Dept. of Bioscience ，Taishan Medical University ，Taian 271016，China ） LIU Ren-min（College of Chemistry and Chemical Engineering，Liaocheng University ，Liaocheng 252059，China ） ABSTRACT OBJECTIVE ：To study and compare the chemical components of the volatile constituents from different parts of Sal- via miltiorrhiza. METHODS ：The volatile oils were extracted from roots ，stems ，leaves and flower of S. miltiorrhiza by steam distil- lation. GC-MS was used to analyze chemical components. The relative content of each component was calculated by area normaliza- tion. RESULTS ：39，33，21 and 28 kinds of chemical compositions were indentified in volatile oil from roots ，stems ，leaves and flowers of S. miltiorrhiza respectively. The main chemical components from different parts of S. miltiorrhiza were different from each other significantly. The main chemical components were quite different except the content of germacrene D in stems ，leaves and flowers took up big proportion. CONCLUSION ：The study p