猪源大肠杆菌脂多糖的提取、纯化及活性分析.pdf

2017 44 3 912919 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j lipopoly saccharides L PS S rDNA LPS DNase RNaseA K LPS Bradford LPS LD LPS ku EU mg LPS LD mg kg L PS L PS S A Extraction Purification and Activity Analysis of Lipopolysaccharides from Escherichia coli Isolated from Swine FENG Jiang WANG Yukun WEI Yuhao YI Qiong WANG Lu XIAN Simei College of A nimal Science Guiz hou Univ ersity Guiy ang China Biochemistry Eng ineering Center of Guiz hou Prov ince Guiy ang China Abstract In order to obtain higher purity and high yield of L PS isolated f rom Esche r ichia coli and evaluate it s virulence ex crement of diarrhea piglet w as collected as pathological samples of w hich some strains of Esche r ichia coli w ere isolated and identified A nd f urthermore a strain of Esche r ichia coli w as acquired by S rDN A identification T he L PS f rom this strain of Esche r ich ia coli w as isolated w ith the hot henol w ater method and purified by DNase RNaseA ro p p teinase K treatment and alcohol precipitation T he poly saccharide rotein and nucleic acid con p tent of purif ied L PS w ere detected by phenolsulf uric acid method Bradf ord method and U V point s spectrophotometric method A nd it s bioactivity and acute median lethal do se w as determi nated by tachy pleus amebocy te ly sate T A L method and acute toxicity ex periment method re spectively T he result s show ed that a strain of h