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L-苏氨酸高产菌株的选育及发酵条件优化研究.PDF

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现代食品科技 Modern Food Science and Technology 2011, Vol.27, No.8 L-苏氨酸高产菌株的选育及发酵条件优化研究 周旭波 (广东环西生物科技股份有限公司,广东揭阳 515343) R - 摘要:采用L-苏氨酸生产菌HXS101(AHV ,Ile )为出发菌株,经NTG 诱变处理,选育出一株 L-苏氨酸高产菌株HXS315 为目的 突变株,在 10 L 发酵罐产酸率最高达到 94.4 g/L (36 h),然后对其进行发酵条件优化,使菌株HXS315 的L-苏氨酸 10 L 发酵罐产酸率提 高到 105.1g/L (36 h)。 n 关键词:苏氨酸;选育;诱变;发酵 c 文章篇号:1673-9078(2011)8-991-994 . Screening and Optimization of Fermentation Conditions of a High-yield m L-Threonine-producing Strain o ZHOU Xu-bo c (Guangdong HUANXI Biotechnology Co., Ltd, Jieyang 515343, China) . R - Abstract: A L-threonine-producing strain HXS101 (AHV ,Ile ) was used as starting strain and induced by NTG to improve its d L-threonine-producing capability. A mutant named as HXS315 was screened, with which the acid production rate reached 94.4 g/L (36 h) in a 10 L o fermentor and improved to 105.1 g/L (36 h) under the optimized fermentation conditions Key words: threonine; breeding; mutation; fermentation o f L-苏氨酸是人体和动物体内的必需氨基酸,自身不 1.1 菌种 n 能合成,必须从食物中摄取[1] 。在人和动物所需的8种必 苏氨酸生产菌HXS101 为广东环西生物科技股份
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