β-葡萄糖苷酶高产菌株选育、发酵条件优化及酶法转化生产龙胆低聚糖的研究的开题报告.docx

β-葡萄糖苷酶高产菌株选育、发酵条件优化及酶法转化生产龙胆低聚糖的研究的开题报告 摘要： β-葡萄糖苷酶是一种重要的生物催化剂，在食品、医药等领域具有广泛应用。本研究旨在选育高产β-葡萄糖苷酶的菌株，优化其发酵条件，并利用该酶法转化生产龙胆低聚糖。首先通过筛选菌株，根据β-葡萄糖苷酶的活力评价其高产能力，并进行深度优化发酵条件，包括培养基成分、培养条件等，最终选定最优的菌株和发酵条件。随后，利用该菌株提取β-葡萄糖苷酶，并对其进行酶学性质的研究，以便于了解β-葡萄糖苷酶催化反应的适宜条件。最后，采用该酶法将可再生材料龙胆草多糖进行低聚糖化，进一步研究其生产工艺和性质。本研究的结果有望提高β-葡萄糖苷酶的生产效率和产量，同时为龙胆低聚糖的工业化生产提供新的方法和途径。 关键词：β-葡萄糖苷酶；菌株选育；发酵条件优化；龙胆低聚糖；酶法转化 Abstract: Beta-glucosidase is an important biocatalyst that finds extensive use in the food, pharmaceutical, and other sectors. The objective of this study was to select a high-yield strain that produces beta-glucosidase, optimize its fermentation conditions, and use the enzyme to produce Gentiana low molecular weight polysaccharide (GLMP) through enzymatic transformation. First, screening strains were undertaken, and the beta-glucosidase activity measured to evaluate its ability to produce high yields. The fermentation conditions, such as the composition of the medium and culture conditions, were deeply optimized based on beta-glucosidase activity. The best strain and fermentation conditions were then selected. The beta-glucosidase was then extracted from the strain, and its enzymatic properties were studied to better understand the optimal conditions for the enzymatic reaction. Finally, the GLMP was transformed into low molecular weight polysaccharides using this enzyme method; process parameters and properties were studied. The study results are anticipated to improve beta-glucosidase production efficiency and yield and provide a new direction and approach for the industrial production of Gentiana low molecular weight polysaccharides. Keywords: beta-glucosidase; strain selection; fermentation condition optimization; Gentiana low molecular weight polysaccharide; enzymatic transformation