combined effects of uvr and temperature on the survival of crab larvae (zoea i) from patagonia the role of uv-absorbing compounds紫外线辐射和温度的蟹幼虫的生存(幼体我)从巴塔哥尼亚防紫外线的作用的化合物.pdf

Mar. Drugs 2010, 8, 1681-1698; doi:10.3390/md8051681 OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Combined Effects of UVR and Temperature on the Survival of Crab Larvae (Zoea I) from Patagonia: The Role of UV-Absorbing Compounds Rodrigo D. Hernández Moresino and E. Walter Helbling * Estación de Fotobiología Playa Unión and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Casilla de Correos N° 15 (9103) Rawson, Chubut, Argentina; E-Mail: efpu@.ar * Author to whom correspondence should be addressed; E-Mail: whelbling@.ar; Tel.: +54-2965-498-019. Received: 22 April 2010; in revised form: 12 May 2010 / Accepted: 21 May 2010 / Published: 25 May 2010 Abstract: The aim of our study was to assess the combined impact of UVR (280–400 nm) and temperature on the first larval stage (Zoea I) of three crab species from the Patagonian coast: Cyrtograpsus altimanus, C. angulatus, and Leucippa pentagona. We determined the survival response of newly hatched Zoea I after being exposed for 8–10 h under a solar simulator (Hönle SOL 1200) at 15 and 20 °C. There was no mortality due to Photosynthetic Active Radiation (PAR, 400–700 nm) or ultraviolet-A radiation (UV-A, 315–400 nm), and all the observed mortality was due to ultraviolet-B radiation (UV-B, 280–315 nm). The data of larval mortality relative to exposure time was best fit using a sigmoid curve. Based on this curve, a threshold (Th) and the lethal dose for 50% mortality