the effect of uv-b radiation on bufo arenarum embryos survival and superoxide dismutase activityuv - b辐射对的影响以arenarum胚胎生存和超氧化物歧化酶活性.pdf

Int. J. Environ. Res. Public Health 2006, 3(1), 43-47 International Journal of Environmental Research and Public Health ISSN 1661-7827 © 2006 by MDPI The Effect of UV-B Radiation on Bufo arenarum Embryos Survival and Superoxide Dismutase Activity 1 1 1 J. Herkovits *, J. L. D´Eramo and O. Fridman 1Programa de Seguridad Química, Instituto de Ciencias Ambientales y Salud, Fundación PROSAMA, Paysandú 752 (1405), Buenos Aires, Argentina. Member of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) de la Argentina. *Correspondence to Dr. Jorge Herkovits. Email: herkovit@mail.retina.ar Received: 05 January 2006 / Accepted: 17 March 2006 / Published: 31 March 2006 Abstract: The exposure of Bufo arenarum embryos to 300-310 nm UV-B at a dose of 4,104 Joule/m2 resulted in 100% lethality within 24 hr while 820 Joule/m2 was the NOEC value for short-term chronic (10 days) exposure. The dose response curves show that lethal effects are proportional with the dose and achieve its highest value within 48 hr post exposure. The superoxide dismutase (SOD) activity in amphibian embryos for sublethal UV-B exposures was evaluated by means of UV-B treatments with 273 (A), 820(B), 1368(C) and 1915(D) Joule/m2 at 2 and 5 hours post irradiation. The SOD