superoxide dismutase as a novel macromolecular nitric oxide carrier preparation and characterization超氧化物歧化酶作为一种新型高分子一氧化氮载体制备和表征.pdf

Int. J. Mol. Sci. 2012, 13, 13985-14001; doi:10.3390/ijms131113985 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Superoxide Dismutase as a Novel Macromolecular Nitric Oxide Carrier: Preparation and Characterization Ssu-Han Chen 1, Shih-Jiuan Chiu 2 and Teh-Min Hu 1,* 1 School of Pharmacy, National Defense Medical Center, Taipei 11490, Taiwan; E-Mail: kido9300630@.tw 2 School of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan; E-Mail: shihjiuan@ * Author to whom correspondence should be addressed; E-Mail: tmhu@.tw; Tel.: +886-2-8792-4868. Received: 28 September 2012; in revised form: 24 October 2012 / Accepted: 25 October 2012 / Published: 29 October 2012 Abstract: Nitric oxide (NO) is an important molecule that exerts multiple functions in biological systems. Because of the short-lived nature of NO, S-nitrosothiols (RSNOs) are believed to act as stable NO carriers. Recently, sulfhydryl (SH) containing macromolecules have been shown to be promising NO carriers. In the present study, we aimed to synthesize and characterize a potential NO carrier based on bovine Cu,Zn-superoxide dismutase (bSOD). To prepare S-nitrosated bSOD, the protein was incubated with S-nitrosoglutathione (GSNO) under varied experimental conditions. The results show that significant S-nitrosation of bSOD occurred on