sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies精子细胞产生不同的细胞因子反应外周单核细胞从不孕妇女血清anti-sperm抗体.pdf

Sperm Cells Induce Distinct Cytokine Response in Peripheral Mononuclear Cells from Infertile Women with Serum Anti-Sperm Antibodies 1 2 3 2 2 Miloslav Kverka , Zdenka Ulcova-Gallova *, Jirina Bartova , Jan Cibulka , Katarina Bibkova , Zdenka Micanova2, Helena Tlaskalova-Hogenova1 1 Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic, 2 Department of Gynecology and Obstetrics, Charles University and Faculty Hospital, Pilsen, Czech Republic, 3 Institute of Dental Research, General University Hospital, First Faculty of Medicine of the Charles University, Prague, Czech Republic Abstract Background and Aims: Anti-sperm antibodies in can markedly reduce the likelihood of natural conception. The etiology of this anti-sperm immunity in human females is unknown. We compared the cytokine response of peripheral blood mononuclear cells (PBMCs) from infertile patients with or without anti-sperm antibodies (ASA) and fertile women. Methodology/Principal Findings: We cultivated the PBMCs together with sperm antigens (whole cells or cell lysate), and screened the supernatants for 40 cytokines by antibody array. When stimulated with whole sperm cells, the PBMCs from patients with ASA produce less IL-3, IL-11, IL-13, ICAM-1, GCSF and more IL-2, IL-4 and IL-12p70 as compared to healthy women. PBMCs from patients with ASA produce typically less IL-13, IL-7, IL-17 and MIG, and more MIP-1b and IL-8, as compared to PBMCs from patients without ASA. In response to sperm cell lysate, PBMCs from infertile women without ASA respond initially by increase in production of growth factors (GCSF, GM-CSF and PDGF-BB) followed by increase in