anti-hiv-1 response elicited in rabbits by anti-idiotype monoclonal antibodies mimicking the cd4-binding siteanti-hiv-1反应引起的兔子anti-idiotype单克隆抗体模仿cd4-binding站点.pdf

Anti-HIV-1 Response Elicited in Rabbits by Anti-Idiotype Monoclonal Antibodies Mimicking the CD4-Binding Site 1 1 1 1 1 Roberto Burioni *, Nicasio Mancini , Donata De Marco , Nicola Clementi , Mario Perotti , Giovanni 1 1 1 2 3 2 Nitti , Monica Sassi , Filippo Canducci , Krisha Shvela , Patrizia Bagnarelli , John R. Mascola , Massimo Clementi1 ` 1 Laboratorio di Microbiologia e Virologia, Universita Vita-Salute San Raffaele, Istituto Scientifico San Raffaele, Diagnostica e Ricerca San Raffaele, Milano, Italia, 2 Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America, 3 Laboratorio di ` Virologia, Universita Politecnica delle Marche, Ancona, Italia Abstract Antibodies against conserved epitopes on HIV-1 envelope glycoproteins (Env), such as the gp120 CD4-binding site (CD4bs), could contribute to protection against HIV-1. Env-based immunogens inducing such a response could be a major component of future anti-HIV-1 strategies. In this proof-of-concept study we describe the generation of two anti-idiotype (AI) murine antibodies mimicking the CD4bs epitope. Sera were collected from long-term non-progressor patients to obtain CD4bs-directed IgG, through sequential purification steps. The purified IgG were then used as Fab fragments to immunize mice for hybridoma generation. Two hybridomas (P1 and P2), reacting only against the CD4bs-directed IgG, were identified and characterized. The P1 and P2