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APOMABH, a La-Specific Monoclonal Antibody, Detects the Apoptotic Tumor Response to Life-Prolonging and DNA-Damaging Chemotherapy 1 1 2 2 3 1,4 Fares Al-Ejeh , Jocelyn M. Darby , Chris Tsopelas , Douglas Smyth , Jim Manavis , Michael P. Brown * 1 Experimental Therapeutics Laboratory, Hanson Institute, Adelaide, South Australia, Australia, 2 Department of Nuclear Medicine, Royal Adelaide Hospital, Adelaide, South Australia, Australia, 3 Centre for Neurological Disease, Hanson Institute, Adelaide, South Australia, Australia, 4 Department of Medical Oncology, Royal Adelaide Hospital Cancer Centre and School of Medicine, The University of Adelaide, Adelaide, South Australia, Australia Abstract Background: Antineoplastic therapy may impair the survival of malignant cells to produce cell death. Consequently, direct measurement of tumor cell death in vivo is a highly desirable component of therapy response monitoring. We have previously shown that APOMABH representing the DAB4 clone of a La/SSB-specific murine monoclonal autoantibody is a malignant cell-death ligand, which accumulates preferentially in tumors in an antigen-specific and dose-dependent manner after DNA-damaging chemotherapy. Here, we aim to image tumor uptake of APOMABH (DAB4) and to define its biological correlates. Methodology/Principal Findings: Brisk tumor cell apoptosis is induced in the syngeneic EL4 lymphoma model after treatment of tumor-bearing mice with DNA-damaging cyclophosphamide/etoposide chemotherapy. Tumor and normal organ accumulation of Indium 111 (111In)-labeled La-specific DAB4 mAb as whole IgG or IgG fragments was quantified by whole-body static im