structural diversity of streptococcal mutans synthesized under different culture and environmental conditions and its effect on mutanase synthesis链球菌变形链球菌合成结构多样性在不同的文化和环境条件及其对mutanase合成的影响.pdf

Molecules 2012, 17, 11800-11815; doi:10.3390/molecules171011800 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Structural Diversity of Streptococcal Mutans Synthesized under Different Culture and Environmental Conditions and Its Effect on Mutanase Synthesis Adrian Wiater *, Małgorzata Pleszczyńska, Katarzyna Próchniak and Janusz Szczodrak Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland; E-Mails: mplesz@poczta.onet.pl (M.P.); kasiaproch@poczta.onet.pl (K.P.); szczo@poczta.umcs.lublin.pl (J.S.) * Author to whom correspondence should be addressed; E-Mail: adrianw2@wp.pl; Tel./Fax: +48-81-537-5960. Received: 14 August 2012; in revised form: 27 September 2012 / Accepted: 28 September 2012 / Published: 9 October 2012 Abstract: Streptococcal mutans synthesized under different conditions by growing cultures or by their glucosyltransferases were shown to exhibit a great structural and property diversity. Culturing and environmental factors causing structural differences in mutans were specified. All of the obtained biopolymers (76 samples) were water-insoluble and most of them (72) had a structure with a predominance of α-(1→3)-linked glucose (i.e., the content of α-(1→3)-linkages in the glucan was always higher than 50%, but did not exceed 76%). An exception were four glucans containing more than 50% of α-(1→6)- sequences. In these structurally unique mutans, the ra