the effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and lfabp in a rat model of nash可可补充在肝脂肪变性的影响,活性氧和lfabp纳什的大鼠模型.pdf

Janevski et al. Comparative Hepatology 2011, 10:10 /content/10/1/10 RESEARCH Open Access The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH 1 2 1 3 1* Mile Janevski , Kiriakos N Antonas , Melanie J Sullivan-Gunn , Maree A McGlynn and Paul A Lewandowski Abstract Background: Non alcoholic steatohepatitis is hypothesised to develop via a mechanism involving fat accumulation and oxidative stress. The current study aimed to investigate if an increase in oxidative stress was associated with changes in the expression of liver fatty acid binding protein in a rat model of non alcoholic steatohepatitis and whether cocoa supplementation attenuated those changes. Methods: Female Sprague Dawley rats were fed a high fat control diet, a high fat methionine choline deficient diet, or one of four 12.5% cocoa supplementation regimes in combination with the high fat methionine choline deficient diet. Results: Liver fatty acid binding protein mRNA and protein levels were reduced in the liver of animals with fatty liver disease when compared to controls. Increased hepatic fat content was accompanied by higher levels of oxidative stress in animals with fatty liver disease when compared to controls. An inverse association was found between the levels of hepatic liver fatty acid binding protein and the level of hepatic oxidative stress in fatty liver disease. Elevated NADPH oxidase protein levels were detected in the liver of animals with increased severity in inflammation and fibrosis. Cocoa supplementation was associated with partial attenuation of these pathological changes, although the severity of liver disease induced by