论肝豆汤对肝豆状核变性皮肤成纤维细胞模型铜代谢的影响（The effect of hepatocellular decoction on the copper metabolism of fibroblast in the skin fibroblast was studied）.doc

论肝豆汤对肝豆状核变性皮肤成纤维细胞模型铜代谢的影响（The effect of hepatocellular decoction on the copper metabolism of fibroblast in the skin fibroblast was studied） The effect of hepatocellular decoction on the copper metabolism of fibroblast in the skin fibroblast was studied Date: May 25 Abstract: abstract: to explore the mechanism of the treatment of hepatocellular degeneration in the treatment of hepatocellular degeneration in the cell level. Methods: to observe the changes of the content of trace elements in the intracellular Cu2 +, Zn2 + and so on of the hepatic bean soup rabbit serum by establishing 23 cases of the fibroblast model of hepatic bean. Results abstract: the content of copper in cells was reduced from (80.94 + 34.76) ng/mg to ng/mg (46.90 + 22.14) ng/mg (P 0.01), and the content of zinc in the cell was increased to (151.43 + + 33.81) ng/mg (P 0.01). Conclusion: hepatocellular decoction has obvious function of lining copper and increasing the content of zinc in cells. Effect of Gandou Decoction on Copper Metabolism of Skin Fibroblast of Hepatolenticular Degeneration Model Objective摘要: To explore the cytological mechanism of Gandou Decoction (GDD) in treating hepatolenticular degeneration (HLD).Methods摘要: Twenty-three in vitro models of cultured skin fibroblast of HLD were established to observe the intracellular change of microelements (Cu2＋， Zn2＋, etc.) content before and after adding rabbit′s serum containing GDD.Results摘要: After being treated with GDD contained serum for 24 hours, the intracellular content of Cu2＋ decreased from (80.94±34.76) ng/mg to (46.90±22.14) ng/mg, P＜0.01, while that of Zn2＋ increased from (140.43±33.81) ng/mg to (151.43±37.83) ng/mg, P＜0.01.Conclusion摘要: GDD is quite effective in removing the intracellular copper and increasing the intracellular zinc. Key words hep atolenticular degeneration, Gandou decoction, model of cultured skin fibroblasts of hepatolenticular degeneration, rabbit serum containing Gandou decoction, intracellular contents