the contribution of 17beta-hydroxysteroid dehydrogenase type 1 to the estradiol-estrone ratio in estrogen-sensitive breast cancer cells的贡献17 beta-hydroxysteroid脱氢酶1型estradiol-estrone比率对于乳腺癌细胞.pdf

The Contribution of 17beta-Hydroxysteroid Dehydrogenase Type 1 to the Estradiol-Estrone Ratio in Estrogen-Sensitive Breast Cancer Cells 1,2. 3. 2 1,4 Chen-Yan Zhang , Jiong Chen , Da-Chuan Yin , Sheng-Xiang Lin * ´ ´ ´ 1 Laboratory of Molecular Endocrinology and Oncology, Centre Hospitalier de Universite Laval Research Center (CHUL, CHUQ) and Laval University, Quebec City, Quebec, Canada, 2 Key Laboratory for Space Bioscience and Biotechnology, Faculty of Life Sciences, Northwestern Polytechnic University, Xi’an, Shaanxi, China, 3 Laboratory of Structural Biology with Visiting Scientists, Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Shanghai, China, 4 WHO Collaborative Center in Human Reproductive Research, Shanghai, China Abstract Estrone and estradiol are both estrogens with estrone being the less potent form and estradiol being the most potent estrogen. The binding of the latter to cellular regulatory elements stimulates the proliferation of breast cancer cells. A high ratio of estradiol/estrone is related to increased cell proliferation, and is of great importance to understanding of breast cancer mechanisms. 17beta-hydroxysteroid dehydrogenase type 1 and type 2 play important roles in the activation of estrone and inactivation of estradiol. Breast cancer cells T47D, MCF-7, BT 20, and JEG 3 as control cells, were chosen to evaluate the contribution of these two enzymes to the ratio. Twenty four hours after addition of different concentrations of estrone and estradiol, the ratio stabilized to around 9/1 in breast cancer cel