the anti-apoptosis effects of daidzein in the brain of d-galactose treated mice大豆苷的抗凋亡作用d-galactose治疗小鼠的大脑.pdf

Molecules 2007, 12, 1455-1470 molecules ISSN 1420-3049 © 2007 by MDPI /molecules Full Paper The Anti-apoptosis Effects of Daidzein in the Brain of D-Galactose Treated Mice Zhen Mao 1, 2,†, Yuan-lin Zheng 1,*, Yan-qiu Zhang 2 , Bao-ping Han 1,2, Xiao-wan Zhu 1, Qing 1 1 Chang and Xiang-bin Hu 1 Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Xuzhou Normal University, Xuzhou 221116, Jiangsu Province, P. R. China; E-mails: zhenmao80@ (Zhen Mao); ylzheng@ (Yuan-lin Zheng) 2 School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, Jiangsu Province, P. R. China * Author to whom correspondence should be addressed; E-mail addresses: ylzheng@, yzheng170@; Tel: (+86) 516 Fax: (+ 86) 516 Received: 30 May 2007; in revised form: 3 July 2007 / Accepted: 13 July 2007 / Published: 19 July 2007 Abstract: The purpose of this study was to explore the neuroprotective effects of daidzein on the apoptotic pathway in the hippocampus and cortex of D-galactose treated mice. For this purpose we have examined the expression of bcl-2 mRNA, bax mRNA and caspase-3 in the hippocampus and cortex of D-galactose-treated mice after fed with 10 or 5 mg/kg of daidzein. The results of in situ hybridization experiments indicate that daidzein could help increase the transcriptions of bcl-2 and decrease the transcriptions of bax in those brain regions of D-galactose-treated