daidzein prevents the increase in cd4+cd28null t cells and b lymphopoesis in ovariectomized mice a key mechanism for anti-osteoclastogenic effect大豆苷防止增加cd4 + cd28null t细胞和b lymphopoesis切除卵巢的老鼠anti-osteoclastogenic效应的一个关键机制.pdf

+ Daidzein Prevents the Increase in CD4 CD28null T Cells and B Lymphopoesis in Ovariectomized Mice: A Key Mechanism for Anti-Osteoclastogenic Effect 1 1 1 2 2 1 Abdul Malik Tyagi , Kamini Srivastava , Kunal Sharan , Dinesh Yadav , Rakesh Maurya , Divya Singh * 1 Division of Endocrinology, Central Drug Research Institute (Council of Scientific and Industrial Research), Chattar Manzil, Lucknow, India, 2 Division of Medicinal and Process Chemistry, Central Drug Research Institute (Council of Scientific and Industrial Research), Chattar Manzil, Lucknow, India Abstract Estrogen deficiency leads to an upregulation of TNF-a producing T cells and B-lymphopoesis which augments osteoclastogenesis. Estrogen deficiency also increases the population of premature senescent CD4+CD28null T cells which secrete a higher amount of TNF-a thus leading to enhanced osteoclastogenesis. Isoflavonoids like daidzein and genistein are found mostly in soybeans, legumes, and peas. These share structural similarity with 17b-stradiol (E2) and have osteoprotective role. This study explores the effect of daidzein (Daid) on the proliferation of TNF-a producing T cells, premature senescent T cells and B cell lymphopoesis under estrogen deficient conditions. For this study adult Balb/c mice were treated with Daid at 10 mg/kg body weight dose by oral gavage daily post ovariectomy (Ovx). After six weeks animals were autopsied and bone marrow and spleen cells were collected for FACS analysis. Blood serum was collected for ELISA. It was observed that Ovx mice treated with Daid for