the alkaloid ageladine a, originally isolated from marine sponges, used for ph-sensitive imaging of transparent marine animals生物碱ageladine,最初从海绵中分离,用于ph-sensitive成像的透明的海洋动物.pdf

Mar. Drugs 2012, 10, 223-233; doi:10.3390/m OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article The Alkaloid Ageladine A, Originally Isolated from Marine Sponges, Used for pH-Sensitive Imaging of Transparent Marine Animals Ulf Bickmeyer Alfred Wegener Institute for Polar and Marine Research in the Helmholtz Society, Am Handelshafen 12, 27570 Bremerhaven, Germany; E-Mail: Ulf.Bickmeyer@awi.de; Tel.: +49-471-4831-2028; Fax: +49-471-4831-1149 Received: 7 December 2011; in revised form: 10 January 2012 / Accepted: 10 January 2012 / Published: 19 January 2012 Abstract: The brominated pyrrole-imidazole Ageladine A was used for live imaging of the jellyfish (jellies) Nausithoe werneri, the sea anemone Metridium senile and the flatworm Macrostomum lignano . The fluorescence properties of Ageladine A allow for estimation of pH values in tissue and organs in living animals. The results showed that Nausithoe werneri had the most acidic areas in the tentacles and close to the mouth (pH 4–6.5), Metridium senile harbours aggregates of high acidity in the tentacles (pH 5) and in Macrostomum lignano , the rhabdoids, the gonads and areas close to the mouth were the most acidic with values down to pH 5. Keywords: confocal microscopy; fluorescence; transparent animal; non invasive 1. Introduction Secondary metabolites from plants and animals have been used for medical or ceremonial treatments and as dyes since ancient times [