the evolving transcriptome of head and neck squamous cell carcinoma a systematic review头颈部鳞状细胞癌演变转录组的一个系统的复习.pdf

The Evolving Transcriptome of Head and Neck Squamous Cell Carcinoma: A Systematic Review Yau-Hua Yu1,2*, Hsu-Ko Kuo3,4*, Kuo-Wei Chang1,2 1 School of Dentistry, National Yang-Ming University, Taipei, Taiwan, 2 Department of Dentistry and the Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan, 3 Department of Geriatrics and Gerontology and the Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan, 4 Division of Gerontology Research, National Health Research Institutes, Taipei, Taiwan Abstract Background: Numerous studies were performed to illuminate mechanisms of tumorigenesis and metastases from gene expression profiles of Head and Neck Squamous Cell Carcinoma (HNSCC). The objective of this review is to conduct a network-based meta-analysis to identify the underlying biological signatures of the HNSCC transcriptome. Methods and Findings: We included 63 HNSCC transcriptomic studies into three specific categories of comparisons: Pre, premalignant lesions v.s. normal; TvN, primary tumors v.s. normal; and Meta, metastatic or invasive v.s. primary tumors. Reported genes extracted from the literature were systematically analyzed. Participation of differential gene activities across three progressive stages deciphered the evolving nature of HNSCC. In total, 1442 genes were verified, i.e. reported at least twice, with ECM1, EMP1, CXCL10 and POSTN shown to be highly reported across all three stages. Knowledge-based networks of the HNSCC transcriptome were constructed, demonstrating integrin signaling and antigen presentation pathways as highly enriched. Notably, functional estimates derived from topological characteristics of integrin signaling networks identified such important genes as ITGA3 and ITG