yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasisyes-associated蛋白表达在头颈部鳞状细胞癌淋巴结的转移.pdf

Yes-Associated Protein Expression in Head and Neck Squamous Cell Carcinoma Nodal Metastasis 2 1 2 3 3 3 4 Lin Ge , Matthew Smail , Wenxia Meng , Yu Shyr , Fei Ye , Kang-Hsien Fan , Xiaohong Li , Hong-Mei 5 1 Zhou *, Neil A. Bhowmick * 1 Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, California, United States of America, 2 State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, China, 3 Department of Biostatistics, Vanderbilt University, Nashville, Tennessee, United States of America, 4 Department of Cancer Biology, Vanderbilt University, Nashville, Tennessee, United States of America, 5 Department of Oral Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China Abstract Introduction: Yes-associated protein (YAP) is considered an oncogene found amplified in multiple tumors, including head and neck squamous cell carcinoma (HNSCC). However, the role for YAP expression in HNSCC is not understood. Based on the central role of YAP in the hippo pathway, we tested if YAP was associated with the stage of HNSCC progression and metastatic potential. Methods: To determine the expression of YAP in human benign and HNSCC tissue specimens, immunohistochemical analyses were performed in whole tissue samples and tissue microarrays. The expression of YAP in tissues of microarray was first associated with clinic-pathologic factors and results verified in samples from whole tissue sections. To investigate the role of YAP and p63 in regulating HNSCC epithelial to mesenchymal transition, epithelial and mesenchymal markers were assayed in Fadu and SCC-25 cells, HNSCC cells with endogenously elevated YAP expression and siRNA-mediated expression knockdown.