the cluster of mir-143 and mir-145 affects the risk for esophageal squamous cell carcinoma through co-regulating fascin homolog 1mir - 143的集群和mir - 145影响食管鳞状细胞癌的风险通过粘住fascin同族体1.pdf

The Cluster of miR-143 and miR-145 Affects the Risk for Esophageal Squamous Cell Carcinoma through Co-Regulating Fascin Homolog 1 1 1 1 1 1 2 2 3 Ran Liu *, Juan Liao , Miao Yang , Jingyi Sheng , Hao Yang , Yi Wang , Enchun Pan , Wei Guo , 1 4 1 Yuepu Pu , Sun Jung Kim *, Lihong Yin 1 Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, China, 2 Huaian Center for Disease Control and Prevention, Huaian, China, 3 The First People’s Hospital of Huaian, Huaian, China, 4 Department of Life Science, Dongguk University-Seoul, Seoul, Korea Abstract MicroRNAs (miRNAs), 18–24 nt non-coding RNAs, are thought to play important roles in cell proliferation, differentiation, apoptosis, and development. Recent studies suggest that some of the known microRNAs map to a single genomic locale within a single polycistronic transcript. But the roles of the cluster remain to be known. In order to understand the role and mechanism of a cluster of miR-143 and miR-145 in esophageal squamous cell carcinoma (ESCC), the association of mature miR-143 and miR-145 expression with the risk for esophageal cancer was evaluated in ESCC patients with a case-control study, and target protein regulated by mature miRNA was analyzed in ESCC cell lines with 39UTR luciferase reporter assay. The expression levels of miR-143 and miR-145 were determined in 110 pairs of esophageal cancer tissues and adjacent normal tissues using real-time reverse transcription PCR. The relative expression of miR-143 and miR-145 were statistically different between cancer tissues and matched controls. The combined expression of miR-143 and miR-145 was significantly