association of single nucleotide polymorphisms of il23r and il17 with ulcerative colitis risk in a chinese han population单核苷酸多态性il23r协会和il17与溃疡性结肠炎的风险在中国汉族人群.pdf

Association of Single Nucleotide Polymorphisms of IL23R and IL17 with Ulcerative Colitis Risk in a Chinese Han Population 1. 1. 1 1 1 2 Pengli Yu , Fangcheng Shen , Xiaofei Zhang , Risheng Cao , Xiaodan Zhao , Pengfei Liu , 3 4 1 1 Huiming Tu , Xiaozhong Yang , Ruihua Shi *, Hongjie Zhang * 1 Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China, 2 Department of Gastroenterology, the People’s Hospital of Jiangyin, Jiangyin, China, 3 Department of Gastroenterology, the Fourth People’s Hospital of Wuxi, Wuxi, China, 4 Department of Gastroenterology, the First Hospital of Huaian, Huaian, China Abstract Background: Previous studies implicated that IL23R and IL17 genes play an important role in autoimmune inflammation. Genome-wide association studies have also identified multiple single nucleotide polymorphisms (SNPs) in the IL23R gene region associated with inflammatory bowel diseases. This study examined the association of IL23R and IL17A gene SNPs with ulcerative colitis susceptibility in a population in China. Methodology: A total of 270 ulcerative colitis and 268 healthy controls were recruited for the analyses of 23 SNPs in the IL23R and IL17A regions. Genomic DNA was extracted and analysis of these 23 SNPs using ligase detection reaction allelic (LDR) technology. Genotype and allele associations were calculated using SPSS 13.0 software package. Principal Findings: Compared to the healthy controls, the variant alleles IL23R rs7530511, and r showed a statistically significant difference for ulcerative colitis susceptibility (0.7% vs 3.3%, P = 0.002; 60.4% vs