conserved expression signatures between medaka and human pigment cell tumors守恒的表达式之间的签名青鳉和人工色素细胞肿瘤.pdf

Conserved Expression Signatures between Medaka and Human Pigment Cell Tumors 1 1 1 1 2 Manfred Schartl *, Susanne Kneitz , Brigitta Wilde , Toni Wagner , Christiaan V. Henkel , Herman P. Spaink3, Svenja Meierjohann1 ¨ ¨ 1 Physiological Chemistry I, Biocenter, University of Wurzburg, Wurzburg, Germany, 2 ZF-Screens B.V., Leiden, The Netherlands, 3 Institute of Biology, Leiden University, Leiden, The Netherlands Abstract Aberrations in gene expression are a hallmark of cancer cells. Differential tumor-specific transcript levels of single genes or whole sets of genes may be critical for the neoplastic phenotype and important for therapeutic considerations or useful as biomarkers. As an approach to filter out such relevant expression differences from the plethora of changes noted in global expression profiling studies, we searched for changes of gene expression levels that are conserved. Transcriptomes from massive parallel sequencing of different types of melanoma from medaka were generated and compared to microarray datasets from zebrafish and human melanoma. This revealed molecular conservation at various levels between fish models and human tumors providing a useful strategy for identifying expression signatures strongly associated with disease phenotypes and uncovering new melanoma molecules. Citation: Schartl M, Kneitz S, Wilde B, Wagner T, Henkel CV, et al. (2012) Conserved Expression Signatures between Medaka and Human Pigment Cell Tumors. PLoS ONE 7(5): e37880. doi:10.1371/journal.pone.0037880 ˜ Editor: Roger Chammas, Faculdade de Medicina, Universidade de Sao Paulo, Braz