complexity of murine cardiomyocyte mirna biogenesis, sequence variant expression and function小鼠心肌细胞microrna的生物起源的复杂性、序列变量表达式和函数.pdf

Complexity of Murine Cardiomyocyte miRNA Biogenesis, Sequence Variant Expression and Function 1 1,2 1,2 1 3 David T. Humphreys , Carly J. Hynes , Hardip R. Patel , Grace H. Wei , Leah Cannon , Diane Fatkin3,4,5, Catherine M. Suter1,4, Jennifer L. Clancy1,2,4*, Thomas Preiss1,2,4,6* 1 Molecular Genetics Division, Victor Chang Cardiac Research Institute, Sydney, New South Wales, Australia, 2 The John Curtin School of Medical Research, The Australian National University, Canberra Australian Capital Territory, Australia, 3 Molecular Cardiology Division, Victor Chang Cardiac Research Institute, Sydney, New South Wales, Australia, 4 St. Vincent’s Clinical School, University of New South Wales, Sydney, New South Wales, Australia, 5 Cardiology Department, St Vincent’s Hospital, Sydney, New South Wales, Australia, 6 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia Abstract microRNAs (miRNAs) are critical to heart development and disease. Emerging research indicates that regulated precursor processing can give rise to an unexpected diversity of miRNA variants. We subjected small RNA from murine HL-1 cardiomyocyte cells to next generation sequencing to investigate the relevance of such diversity to cardiac biology. ,40 million tags were mapped to known miRNA hairpin sequences as deposited in miRBase version 16, calling 403 generic miRNAs as appreciably expressed. Hairpin arm bias broadly agreed with miRBase annotation, although 44 miR* were unexpectedly abundant (.20% of tags); conversely, 33 -5p/-3p annotated hairpins were asymmetrically expressed. Overall, variability was infrequent at the 59 start but commo