association of group b streptococcus colonization and bovine exposure a prospective cross-sectional cohort studyb群链球菌殖民协会和牛接触未来的横断面队列研究.pdf

Association of Group B Streptococcus Colonization and Bovine Exposure: A Prospective Cross-Sectional Cohort Study Shannon D. Manning1,2,3, A. Cody Springman1,2,3, Amber D. Million1,2, Nicole R. Milton1,2,3, Sara E. 4 4 2 3 McNamara , Patricia A. Somsel , Paul Bartlett , H. Dele Davies * 1 Microbial Evolution Laboratory, Michigan State University, East Lansing, Michigan, United States of America, 2 National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan, United States of America, 3 Department of Pediatrics and Human Development, Michigan State University, East Lansing, Michigan, United States of America, 4 Bureau of Laboratories, Michigan Department of Community Health, Lansing, Michigan, United States of America Abstract Background: While Group B Streptococcus (GBS) human colonization and infection has long been suspected as originating from cows, several investigators have suggested that ongoing interspecies GBS transmission is unlikely due to genotyping data demonstrating that human and bovine-derived GBS strains represent mostly distinct populations. The possibility of ongoing transmission between humans and their livestock has not been systematically examined. Methodology/Principal Findings: To examine ongoing interspecies transmission, we conducted a prospective cross- sectional cohort study of 68 families and their livestock. Stool specimens were collected from 154 people and 115 livestock; GBS was detected in 19 (12.3%) humans and 2 (1.7%) animals (bovine and sheep). Application of multilocus sequence typing (MLST) identified 8 sequence types (STs or clones), with STs 1 and 23 predominating. There were 11 families in which two members submitted stools and at least one had GBS colonizatio