de novo polymerase activity and oligomerization of hepatitis c virus rna-dependent rna-polymerases from genotypes 1 to 5新创聚合酶活性和低聚的丙型肝炎病毒依赖rna的rna-polymerases基因型1 - 5所示.pdf

De Novo Polymerase Activity and Oligomerization of Hepatitis C Virus RNA-Dependent RNA-Polymerases from Genotypes 1 to 5 1. ´ ´ 1,2. ´ ´ 1 ´ Pilar Clemente-Casares , Alberto J. Lopez-Jimenez , Itxaso Bellon-Echeverrıa , Jose Antonio 4 ´ 2 ´ 3 1 Encinar , Elisa Martınez-Alfaro , Ricardo Perez-Flores , Antonio Mas * ´ 1 Centro Regional de Investigaciones Biomedicas (CRIB), Universidad de Castilla La Mancha, Albacete, Spain, 2 Infectious Disease Unit, Complejo Hospitalario Universitario ´ de Albacete, Albacete, Spain, 3 Digestive Department, Complejo Hospitalario Universitario de Albacete, Albacete, Spain, 4 Instituto de Biologıa Molecular y Celular, ´ Universidad Miguel Hernandez, Elche, Spain Abstract Hepatitis C virus (HCV) shows a great geographical diversity reflected in the high number of circulating genotypes and subtypes. The response to HCV treatment is genotype specific, with the predominant genotype 1 showing the lowest rate of sustained virological response. Virally encoded enzymes are candidate targets for intervention. In particular, promising antiviral molecules are being developed to target the viral NS3/4A protease and NS5B polymerase. Most of the studies with the NS5B polymerase have been done with genotypes 1b and 2a, whilst information about other ge