temporal proteome and lipidome profiles reveal hepatitis c virus-associated reprogramming of hepatocellular metabolism and bioenergetics颞蛋白质组和lipidome资料揭示丙型肝炎病毒相关肝细胞代谢的改变和生物能疗法.pdf

Temporal Proteome and Lipidome Profiles Reveal Hepatitis C Virus-Associated Reprogramming of Hepatocellular Metabolism and Bioenergetics 1. 2.¤a 3 3 Deborah L. Diamond *, Andrew J. Syder , Jon M. Jacobs , Christina M. Sorensen , Kathie-Anne 1¤b 1 4 3 3 3 Walters , Sean C. Proll , Jason E. McDermott , Marina A. Gritsenko , Qibin Zhang , Rui Zhao , 3 3 4 3 2 Thomas O. Metz , David G. Camp II , Katrina M. Waters , Richard D. Smith , Charles M. Rice , Michael G. Katze1,5 1 Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington, United States of America, 2 Laboratory of Virology Infectious Disease, Center for the Study of Hepatitis C, Rockefeller University, New York, New York, United States of America, 3 Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America, 4 Computational Biology Bioinformatics, Pacific Northwest National Laboratory, Richland, Washington, United States of America, 5 Washington National Primate Research Center, University of Washington Seattle, Washington, United States of America Abstract Proteomic and lipidomic profiling was performed over a time course of acute hepatitis C virus (HCV) infection in cultured Huh-7.5 cells to gain new insights into the intracellular processes influenced by this virus. Our proteomic data suggest that HCV induces early perturbations in glycolysis, the pentose phosphate pathway, and the