Development and validation of a liquid chromatography–tandem mass spectrometric assay.pdf
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Journal of Chromatography B, 865 (2008) 127–132
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Journal of Chromatography B
j o u rn al h omepage: www.elsevi er.c om /locate/c hromb
Development and validation of a liquid chromatography–tandem mass
spectrometric assay for pitavastatin and its lactone in human plasma and urine
Lei Tian, Yiling Huang, Youhong Jia, Lu Hua, Yishi Li ∗
The Key Laboratory of Clinical Trial Research in Cardiovascular Drugs, Ministry of Health, Fu Wai Hospital, CAMS PUMC, 167 Beilishi Road, Beijing 100037, PR China
a r t i c l e i n f o a b s t r a c t
Article history: A rapid, selective and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method
Received 13 December 2007 with electrospray ionization (ESI) was developed and validated for the simultaneous determination of
Accepted 24 February 2008 pitavastatin and its lactone in human plasma and urine. Following a liquid–liquid extraction, both the
Available online 29 February 2008
analytes and internal standard racemic i-prolact were separated on a BDS Hypersil C8 column, using
methanol–0.2% acetic acid in water (70: 30, v/v) as the mobile phase. The mass spectrometer was operated
Keywords:
in multiple reaction monitoring (MRM) mode using the transition m/z 422.4 → m/z 290.3 for pitavastatin,
Pitavastatin
Pitavastatin lactone m/z 404.3 → m/z 290.3 for pitavastatin lactone and m/z 406.3 → m/z 318.3 for the
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