文档详情

Development and validation of a liquid chromatography–tandem mass spectrometric assay.pdf

发布:2017-05-22约4.27万字共6页下载文档
文本预览下载声明
Journal of Chromatography B, 865 (2008) 127–132 Contents lists available at ScienceDirect Journal of Chromatography B j o u rn al h omepage: www.elsevi er.c om /locate/c hromb Development and validation of a liquid chromatography–tandem mass spectrometric assay for pitavastatin and its lactone in human plasma and urine Lei Tian, Yiling Huang, Youhong Jia, Lu Hua, Yishi Li ∗ The Key Laboratory of Clinical Trial Research in Cardiovascular Drugs, Ministry of Health, Fu Wai Hospital, CAMS PUMC, 167 Beilishi Road, Beijing 100037, PR China a r t i c l e i n f o a b s t r a c t Article history: A rapid, selective and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method Received 13 December 2007 with electrospray ionization (ESI) was developed and validated for the simultaneous determination of Accepted 24 February 2008 pitavastatin and its lactone in human plasma and urine. Following a liquid–liquid extraction, both the Available online 29 February 2008 analytes and internal standard racemic i-prolact were separated on a BDS Hypersil C8 column, using methanol–0.2% acetic acid in water (70: 30, v/v) as the mobile phase. The mass spectrometer was operated Keywords: in multiple reaction monitoring (MRM) mode using the transition m/z 422.4 → m/z 290.3 for pitavastatin, Pitavastatin Pitavastatin lactone m/z 404.3 → m/z 290.3 for pitavastatin lactone and m/z 406.3 → m/z 318.3 for the
显示全部
相似文档