assessment of the n-pcr assay in diagnosis of pleural tuberculosis detection of m.tuberculosis in pleural fluid and sputum collected in tandem评估n-pcr化验的胸膜结核的诊断检测。.pdf

Assessment of the N-PCR Assay in Diagnosis of Pleural Tuberculosis: Detection of M.tuberculosis in Pleural Fluid and Sputum Collected in Tandem 1 2 3 4 5 Parameet Kumar , Manas K. Sen , Devendra S. Chauhan , Vishwa M. Katoch , Sarman Singh , Hanumanthappa K. Prasad1* 1 Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India, 2 Department of Pulmonary Critical Care and Sleep Medicine, Vardhman Mahavir Medical College (VMMC) and Safdarjung Hospital, New Delhi, India, 3 National JALMA Institute of Leprosy and Other Mycobacterial Diseases, Tajganj, Agra, India, 4 Department of Health Research (Ministry of Health Family Welfare) and Indian Council of Medial Research, Ramalingaswami Bhawan, Ansari Nagar, New Delhi, India, 5 Division of Clinical Microbiology, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India Abstract Background: The nonspecific clinical presentation and paucibacillary nature of tuberculous pleuritis remains a challenge for diagnosis. Diagnosis of tuberculous pleural effusion depends on the demonstration of the presence of tubercle bacilli in the sputum, pleural fluid, or pleural biopsy specimen, or demonstration of granuloma in pleura by histological examination. We examined the clinical utility of the diagnosis of pleural tuberculosis using the in house N-PCR assay, AFB smear microscopy and culture. Besides pleural fluid the inclusion of sputum in the efficacy of diagnosis of pleural tuberculosis was scrutinized. Methodology/Principal Findings: Pleural fluid and sputum samples of 58 tuberculous and 42 non-tuberculous pleural effusion patients were processed for AFB smear microscopy, culture and the N-PCR assay. Mycobacteria wer