the development and application of a dot-elisa assay for diagnosis of southern rice black-streaked dwarf disease in the fielddot-elisa分析的发展和应用为黑条矮缩病的诊断.pdf

Viruses 2012, 4, 167-183; doi:10.3390/v4010167 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article The Development and Application of a Dot-ELISA Assay for Diagnosis of Southern Rice Black-Streaked Dwarf Disease in the Field 1 1 1 2 1 1 Zhenchao Wang , Dandan Yu , Xiangyang Li , Mengjiao Zeng , Zhuo Chen , Liang Bi , Jiaju Liu 1, Linhong Jin 1, Deyu Hu 1, Song Yang 1,* and Baoan Song 1,* 1 State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/ Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, P. R. China; E-Mails: gs.zcwang@ (Z.C.W.); dandanyu0813@ (D.D.Y.); gs.lixy09@ (X.Y.L.); fcc.zchen@ (Z.C.); gs.lbi09@ (L.B.); liujiaju 1987@126.com (J.J.L.); fcc.jinlh@ (L.H.J.); fcc.dyhu@ (D.Y.H.) 2 School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510640, P. R. China; E-Mail: 1094185612@ (M.Z.) * Author to whom correspondence should be addressed; E-Mail: songbaoan22@ (B.A.S.); fcc.syang@ (S.Y.); Tel.: +0851-3620521 (B.A.S.); +0851-8292171 (S.Y.); Fax: +0851-3622211. Received: 13 December 2011; in revised form: 5 January 2012 / Accepted: 12 Jan 2012 / Published: 23 January 2012 Abstract: Outbreaks of the southern rice black-streaked dwarf virus (SRBSDV) have caused significant crop losses in southern China in recent years, especially in 2010. There are no effective, quick and pract