synthesis and antiproliferative activity of ammonium and imidazolium ionic liquids against t98g brain cancer cells合成和抗增殖活性和imidazolium铵离子液体对t98g脑癌细胞.pdf

Molecules 2012, 17, 13727-13739; doi:10.3390/molecules171213727 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Synthesis and Antiproliferative Activity of Ammonium and Imidazolium Ionic Liquids against T98G Brain Cancer Cells Nagendra Kumar Kaushik, Pankaj Attri, Neha Kaushik and Eun Ha Choi * Plasma Bioscience Research Center, Kwangwoon University, Seoul 139701, Korea; E-Mails: kaushik.nagendra@kw.ac.kr (N.K.K.); chem.pankaj@ (P.A.); neha.bioplasma@ (N.K.) * Author to whom correspondence should be addressed; E-Mails: ehchoi@kw.ac.kr or Kaushik.nagendra@; Tel.: +82-10-2240-5236; Fax: +82-2-940-5664. Received: 22 October 2012; in revised form: 13 November 2012 / Accepted: 14 November 2012 / Published: 22 November 2012 Abstract: Four ammonium and imidazolium ionic liquids (ILs) have been synthesized and screened against the T98G cell line (brain cancer) and HEK normal cells. Treatment induced metabolic cell death (MTT), growth inhibition, clonogenic inhibition were studied as cellular response parameters. Treatment with ILs enhanced growth inhibition and cell death in a concentration dependent manner in both the T98G and HEK cell lines. At higher concentrations (0.09 mg/mL) the cytotoxic effects of the ILs were highly significant. An inhibitory effect on clonogenic capacity was also observed after cell treatment. Amongst all ILs; IL 4 (BMIMCl) exhibited potent activity against T98G brain cancer cells. Despite potent in-vitro activity, all ILs exhibited less cytotoxicity against the normal human HEK